Many thanks to Turtle Mommy and everyone else for your interest. This is a great forum and I am glad I found you. I apologize for my error regarding our pond volume, the correct size is 400 gallons, occupied by 12 koi each 9-10 inches. Here are some actual correct (!!) facts about our pond. This may be Too Much Information, but here goes...
The pond was built and stocked prior to our 2003 purchase of the property and house. There were eight or so koi at that time, I believe they were a bit smaller than they are now. There have been a few births since then. In 2010 the concrete pond developed a leak and lost over half the water. We transferred the fish and the remaining water into a big laundry tub--where they stayed for at least six months until we had the pond repaired and lined. Now the pond has a polyurea coating. We transferred the fish back into the pond in 2011. We took chemistry readings at that time, the last reading was August 2011: pH 7.4-7.6, ammonia less than .25 ppm, nitrite zero, nitrate 20 ppm.
The current readings are pH 7.8-8.0, ammonia less than .25 ppm, nitrite zero, nitrate 40 ppm. So the pH is a little high. We did not take any readings between 2011 and now.
The only chemicals we have introduced in the past four years are ALGAEFIX and ECOFIX, which we apply during March-May (not every year, just as needed) to control algae blooms. We used the minimum dosages to restore clear water. We did not keep records of applications/exact dosages.
Before and After photos of koi are attached. Thanks again for your suggestions! -- Evie