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Message: [QUOTE="Mucky_Waters, post: 294039, member: 3859"] Just to clarify, although the WWTPs that I was speaking did have an initial settlement tank with some anoxic bacteria action going on in the activated sludge at the bottom of that initial tank, the plants mainly used a series of heavily aerated tanks utilizing [U]aerobic[/U] free floating bacteria as the principal nitrifiers. There was no solid media utilized in these tanks for these bacteria to attach themselves to, so they were most definitely "planktonic", and aerobic. As I say this doesn't really apply to Wayne's situation as the bacteria process employed in these aeration tanks likely would not be very effective at the temps he is dealing with. [/QUOTE]